P22314: Ubiquitin-like modifier-activating enzyme 1

General Information

Protein names	- Ubiquitin-like modifier-activating enzyme 1 - 6.2.1.45 {ECO:0000305\|PubMed:1447181} - Protein A1S9 - Ubiquitin-activating enzyme E1
Gene names	UBA1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P22314

31 N-termini

19 C-termini

14 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSSSPLSKKR RVSGPDPKPG SNCSPAQSVL SEVPSVPTNG MAKNGSEADI DEGLYSRQLY
        70         80         90        100        110        120
VLGHEAMKRL QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTAQWA DLSSQFYLRE
       130        140        150        160        170        180
EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSGFQ VVVLTNTPLE DQLRVGEFCH
       190        200        210        220        230        240
NRGIKLVVAD TRGLFGQLFC DFGEEMILTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH
       250        260        270        280        290        300
GFESGDFVSF SEVQGMVELN GNQPMEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK
       310        320        330        340        350        360
ISFKSLVASL AEPDFVVTDF AKFSRPAQLH IGFQALHQFC AQHGRPPRPR NEEDAAELVA
       370        380        390        400        410        420
LAQAVNARAL PAVQQNNLDE DLIRKLAYVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI
       430        440        450        460        470        480
MQWLYFDALE CLPEDKEVLT EDKCLQRQNR YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG
       490        500        510        520        530        540
CELLKNFAMI GLGCGEGGEI IVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN
       550        560        570        580        590        600
PHIRVTSHQN RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT
       610        620        630        640        650        660
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA
       670        680        690        700        710        720
ENVNQYLTDP KFVERTLRLA GTQPLEVLEA VQRSLVLQRP QTWADCVTWA CHHWHTQYSN
       730        740        750        760        770        780
NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVNNPL HLDYVMAAAN LFAQTYGLTG
       790        800        810        820        830        840
SQDRAAVATF LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP
       850        860        870        880        890        900
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDIPSADR HKSKLIAGKI IPAIATTTAA
       910        920        930        940        950        960
VVGLVCLELY KVVQGHRQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV
       970        980        990       1000       1010       1020
QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS
      1030       1040       1050
RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR

Isoforms

- Isoform 2 of Ubiquitin-like modifier-activating enzyme 1

Sequence View

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Protein Neighborhood

Domains & Features

31 N-termini - 19 C-termini - 14 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P22314-2-unknown	MSSSPL...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P22314-2-Acetylation	SSSPLS...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P22314-2-Acetylation	SSSPLS...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P22314-2-Acetylation	SSSPLS...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P22314-42-acetylation	AKNGSE...	42	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-42-unknown	AKNGSE...	42		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14032
P22314-45-	GSEADI...	45			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P22314-45-	GSEADI...	45			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P22314-45-	GSEADI...	45			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-46-	SEADID...	46			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-70-acetylation	LQTSSV...	70	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-72-	TSSVLV...	72			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-259-unknown	LNGNQP...	259		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18904
P22314-261-unknown	GNQPME...	261		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5689
P22314-305-	SLVASL...	305			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P22314-305-	SLVASL...	305			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P22314-305-	SLVASL...	305			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P22314-309-unknown	SLAEPD...	309		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3697
P22314-310-unknown	LAEPDF...	310		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3698
P22314-351-	NEEDAA...	351			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-356-unknown	AELVAL...	356		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18905
P22314-369-	ALPAVQ...	369			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P22314-372-	AVQQNN...	372			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P22314-375-unknown	QNNLDE...	375		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7367
P22314-399-unknown	AFIGGL...	399		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5690
P22314-403-unknown	GLAAQE...	403		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3699
P22314-476-unknown	AGAIGC...	476		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7368
P22314-643-acetylation	HTLQWA...	643	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-682-unknown	TQPLEV...	682		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17488
P22314-793-	SVQVPE...	793			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P22314-793-	SVQVPE...	793			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P22314-793-	SVQVPE...	793			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P22314-793-	SVQVPE...	793			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P22314-816-unknown	SANASV...	816		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7369
P22314-831-	ATLPSP...	831			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P22314-831-	ATLPSP...	831			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P22314-831-	ATLPSP...	831			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P22314-833-unknown	LPSPDK...	833		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5691
P22314-844-	MYPIDF...	844			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P22314-844-	MYPIDF...	844			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P22314-860-unknown	DFIVAA...	860		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18906
P22314-1011-	LDQPMT...	1011			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P22314-1011-acetylation	LDQPMT...	1011	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...PTNGMA	41	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC14032
...SYVPVT	144	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000404796
...SYVPVT	144	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000474110
...FLSGFQ	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000397816
...FLSGFQ	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000474594
...CHNRGI	183	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000401101
...CHNRGI	183	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000474561
...QGMVEL	258	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18904
...MVELNG	260	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5689
...EIKVLG	270	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000415033
...EIKVLG	270	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000389583
...EIKVLG	270	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000473741
...EIKVLG	270	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000473977
...KSLVAS	308	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3697
...SLVASL	309	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3698
...NEEDAA	355	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18905
...LPAVQQ	374	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7367
...LAPINA	398	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5690
...NAFIGG	402	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3699
...YFLVGA	475	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7368
...LRLAGT	681	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17488
...DQELQS	815	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7369
...ELKATL	832	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5691
...SNFHMD	859	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18906
...RYTIR	1058	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	41	NGMA.\|.AKNG	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
MEP1B_HUMAN	258	MVEL.\|.LNGN	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	260	ELNG.\|.GNQP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	308	LVAS.\|.SLAE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	309	VASL.\|.LAEP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	355	EDAA.\|.AELV	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	374	AVQQ.\|.QNNL	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	398	PINA.\|.AFIG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	402	FIGG.\|.GLAA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	475	LVGA.\|.AGAI	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	681	LAGT.\|.TQPL	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	815	ELQS.\|.SANA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	832	KATL.\|.LPSP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	859	FHMD.\|.DFIV	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)