P13639: Elongation factor 2

General Information

Protein names	- Elongation factor 2 - EF-2
Gene names	EEF2
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P13639

45 N-termini

25 C-termini

27 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MVNFTVDQIR AIMDKKANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR AGETRFTDTR
        70         80         90        100        110        120
KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGAGFLI NLIDSPGHVD FSSEVTAALR
       130        140        150        160        170        180
VTDGALVVVD CVSGVCVQTE TVLRQAIAER IKPVLMMNKM DRALLELQLE PEELYQTFQR
       190        200        210        220        230        240
IVENVNVIIS TYGEGESGPM GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG
       250        260        270        280        290        300
EGQLGPAERA KKVEDMMKKL WGDRYFDPAN GKFSKSATSP EGKKLPRTFC QLILDPIFKV
       310        320        330        340        350        360
FDAIMNFKKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL LQMITIHLPS
       370        380        390        400        410        420
PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK MVPTSDKGRF YAFGRVFSGL
       430        440        450        460        470        480
VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV
       490        500        510        520        530        540
KTGTITTFEH AHNMRVMKFS VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE
       550        560        570        580        590        600
SGEHIIAGAG ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN
       610        620        630        640        650        660
RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN
       670        680        690        700        710        720
ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ
       730        740        750        760        770        780
IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP
       790        800        810        820        830        840
MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK
       850
RKGLKEGIPA LDNFLDKL

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

45 N-termini - 25 C-termini - 27 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P13639-1-unknown	MVNFTV...	1		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P13639-1-unknown	MVNFTV...	1		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P13639-1-	MVNFTV...	1		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P13639-1-	MVNFTV...	1		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-1-	MVNFTV...	1		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-2-unknown	VNFTVD...	2	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P13639-2-unknown	VNFTVD...	2	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16805
P13639-2-unknown	VNFTVD...	2		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P13639-2-unknown	VNFTVD...	2		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P13639-2-unmodified	VNFTVD...	2		other		CNRS/ISV	Large_scale_NTA_Human
P13639-2-	VNFTVD...	2		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P13639-2-	VNFTVD...	2		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-2-	VNFTVD...	2		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-2-	VNFTVD...	2		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P13639-2-	VNFTVD...	2		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-2-	VNFTVD...	2		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P13639-23-	SVIAHV...	23		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-23-	SVIAHV...	23		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-80-unknown	ELSEND...	80	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5586
P13639-81-unknown	LSENDL...	81	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5587
P13639-91-unknown	QSKDGA...	91	inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P13639-107-	GHVDFS...	107		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-137-unknown	VQTETV...	137	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5588
P13639-167-unknown	LQLEPE...	167	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5589
P13639-236-	FAAKGE...	236		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P13639-236-	FAAKGE...	236		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P13639-236-	FAAKGE...	236		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P13639-236-	FAAKGE...	236		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P13639-273-	FSKSAT...	273		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P13639-273-	FSKSAT...	273		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P13639-273-	FSKSAT...	273		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P13639-276-	SATSPE...	276		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P13639-276-	SATSPE...	276		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-276-	SATSPE...	276		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-276-	SATSPE...	276		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-276-	SATSPE...	276		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-372-	LYEGPP...	372		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-372-	LYEGPP...	372		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-372-	LYEGPP...	372		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P13639-372-	LYEGPP...	372		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-375-unknown	GPPDDE...	375	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5590
P13639-401-	MVPTSD...	401		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-431-unknown	GPNYTP...	431	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7308
P13639-431-unknown	GPNYTP...	431	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5591
P13639-500-	SVSPVV...	500		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-507-unknown	VAVEAK...	507		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P13639-507-	VAVEAK...	507		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-541-unknown	SGEHII...	541	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11200
P13639-542-unknown	GEHIIA...	542	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11201
P13639-563-unknown	EDHACI...	563	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7309
P13639-564-unknown	DHACIP...	564	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18716
P13639-573-	SDPVVS...	573		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-606-	ARPFPD...	606		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-606-	ARPFPD...	606		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-612-unknown	GLAEDI...	612	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11202
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13639-612-	GLAEDI...	612		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-615-unknown	EDIDKG...	615	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7310
P13639-616-unknown	DIDKGE...	616	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18717
P13639-616-unknown	DIDKGE...	616	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17325
P13639-617-unknown	IDKGEV...	617	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17326
P13639-637-unknown	EKYEWD...	637	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7311
P13639-637-unknown	EKYEWD...	637	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5592
P13639-652-unknown	FGPDGT...	652	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7312
P13639-656-	GTGPNI...	656		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-717-	GGGQII...	717		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-768-	GHVFEE...	768		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13639-768-	GHVFEE...	768		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P13639-768-	GHVFEE...	768		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-772-unknown	EESQVA...	772	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18718
P13639-773-unknown	ESQVAG...	773	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18719
P13639-775-unknown	QVAGTP...	775	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18720
P13639-786-	AYLPVN...	786		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13639-786-	AYLPVN...	786		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13639-798-unknown	ADLRSN...	798	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5593
P13639-819-unknown	ILPGDP...	819	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5594
P13639-826-unknown	DNSSRP...	826	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18721

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...ISLFYE	79	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5586
...SLFYEL	80	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5587
...VSGVCV	136	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5588
...RALLEL	166	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5589
...ELLYEG	374	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5590
...KVRIMG	430	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7308
...KVRIMG	430	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5591
...CIIEES	540	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11200
...IIEESG	541	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11201
...LKDLEE	562	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7309
...KDLEED	563	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18716
...RPFPDG	611	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11202
...PDGLAE	614	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7310
...DGLAED	615	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18717
...DGLAED	615	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17325
...GLAEDI	616	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17326
...ARYLAE	636	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7311
...ARYLAE	636	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5592
...RKIWCF	651	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7312
...RGHVFE	771	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18718
...GHVFEE	772	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18719
...VFEESQ	774	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18720
...SFGFTA	797	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5593
...FDHWQI	818	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5594
...PGDPFD	825	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18721
...NFLDKL	857	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8223
...FLDKL	858	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...FLDKL	858		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...FLDKL	858		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...FLDKL	858		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...FLDKL	858		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MAP2_HUMAN	1	.\|.VNFT	inferred from experiment	unknown	MEROPS	Wang J	Warder SE et al.:Discovery, identification, and ... (M24.002)	18828628,
CATL1_HUMAN	79	LFYE.\|.ELSE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	80	FYEL.\|.LSEN	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	136	GVCV.\|.VQTE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	166	LLEL.\|.LQLE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	374	LYEG.\|.GPPD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	430	RIMG.\|.GPNY	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	430	RIMG.\|.GPNY	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	540	IEES.\|.SGEH	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAB_HUMAN	541	EESG.\|.GEHI	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CATS_HUMAN	562	DLEE.\|.EDHA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	563	LEED.\|.DHAC	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
GRAB_HUMAN	611	FPDG.\|.GLAE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CATS_HUMAN	614	GLAE.\|.EDID	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	615	LAED.\|.DIDK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	615	LAED.\|.DIDK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	616	AEDI.\|.IDKG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	636	YLAE.\|.EKYE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	636	YLAE.\|.EKYE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	651	IWCF.\|.FGPD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	771	HVFE.\|.EESQ	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	772	VFEE.\|.ESQV	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	774	EESQ.\|.QVAG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	797	GFTA.\|.ADLR	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	818	HWQI.\|.ILPG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	825	DPFD.\|.DNSS	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CBPA4_HUMAN	857	LDKL.\|.L	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)