P01009: Alpha-1-antitrypsin

General Information

Protein names	- Alpha-1-antitrypsin - Alpha-1 protease inhibitor - Alpha-1-antiproteinase - Serpin A1 - Short peptide from AAT - SPAAT
Gene names	SERPINA1
Organism	Homo sapiens
Protease Family
Protease ID	I04.001
Chromosome location
UniProt ID	P01009

24 N-termini

10 C-termini

25 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Alpha-1-antitrypsin - Isoform 3 of Alpha-1-antitrypsin

Sequence View

        10         20         30         40         50         60
MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI TPNLAEFAFS
        70         80         90        100        110        120
LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI LEGLNFNLTE IPEAQIHEGF
       130        140        150        160        170        180
QELLRTLNQP DSQLQLTTGN GLFLSEGLKL VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ
       190        200        210        220        230        240
INDYVEKGTQ GKIVDLVKEL DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV
       250        260        270        280        290        300
KVPMMKRLGM FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL
       310        320        330        340        350        360
ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP LKLSKAVHKA
       370        380        390        400        410
VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMIE QNTKSPLFMG KVVNPTQK         10         20         30         40         50         60
MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI TPNLAEFAFS
        70         80         90        100        110        120
LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI LEGLNFNLTE IPEAQIHEGF
       130        140        150        160        170        180
QELLRTLNQP DSQLQLTTGN GLFLSEGLKL VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ
       190        200        210        220        230        240
INDYVEKGTQ GKIVDLVKEL DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV
       250        260        270        280        290        300
KVPMMKRLGM FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL
       310        320        330        340        350        360
ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP LKLSKAVHKA
       370        380        390        400        410
VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMIE QNTKSPLFMG KVVNPTQK

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Evidence Codes:

Methodology:

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Protease Assignment Confidence:

Evidence Names:

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Protein Neighborhood

Domains & Features

24 N-termini - 10 C-termini - 25 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P01009-1-unknown	MPSSVS...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt65850
P01009-1-unknown	MPSSVS...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt65851
P01009-25-unknown	EDPQGD...	25	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P01009-25-unknown	EDPQGD...	25	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt113830
P01009-25-unknown	EDPQGD...	25	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt113831
P01009-35-unknown	TDTSHH...	35	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC31932
P01009-35-unknown	TDTSHH...	35	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt137987
P01009-35-unknown	TDTSHH...	35	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt137988
P01009-86-unknown	AMLSLG...	86		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-86-unknown	AMLSLG...	86	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180058
P01009-86-unknown	AMLSLG...	86	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180059
P01009-224-unknown	VKDTEE...	224	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19982
P01009-224-unknown	VKDTEE...	224	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147501
P01009-224-unknown	VKDTEE...	224	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147502
P01009-247-unknown	RLGMFN...	247		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-247-unknown	RLGMFN...	247	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180061
P01009-247-unknown	RLGMFN...	247	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180062
P01009-258-unknown	KLSSWV...	258		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-258-unknown	KLSSWV...	258	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180064
P01009-258-unknown	KLSSWV...	258	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180065
P01009-307-unknown	SASLHL...	307		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-307-unknown	SASLHL...	307	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180067
P01009-309-unknown	SLHLPK...	309		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-309-unknown	SLHLPK...	309	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180069
P01009-316-unknown	SITGTY...	316		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-316-unknown	SITGTY...	316	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180071
P01009-325-unknown	SVLGQL...	325		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-325-unknown	SVLGQL...	325	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180073
P01009-335-unknown	VFSNGA...	335		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-335-unknown	VFSNGA...	335	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt180075
P01009-339-unknown	GADLSG...	339		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-343-unknown	SGVTEE...	343		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-356-unknown	AVHKAV...	356		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-360-	AVLTID...	360		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P01009-360-unknown	AVLTID...	360		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-375-unknown	MFLEAI...	375	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19948
P01009-375-unknown	MFLEAI...	375		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-376-unknown	FLEAIP...	376		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19985
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC25108
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21825
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21876
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC23957
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21714
P01009-377-unknown	LEAIPM...	377	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC24789
P01009-379-unknown	AIPMSI...	379	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5345
P01009-379-unknown	AIPMSI...	379		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19986
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC25109
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21826
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC23658
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21877
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC23958
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC21715
P01009-382-unknown	MSIPPE...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC24790
P01009-383-unknown	SIPPEV...	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13911
P01009-383-unknown	SIPPEV...	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8632
P01009-383-unknown	SIPPEV...	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC31935
P01009-383-unknown	SIPPEV...	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5346
P01009-383-unknown	SIPPEV...	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16119
P01009-383-unknown	SIPPEV...	383		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P01009-397-unknown	LMIEQN...	397	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC23959
P01009-397-unknown	LMIEQN...	397		unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...DAAQKT	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31932
...DAAQKT	34	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt120997
...DAAQKT	34	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt120999
...ERPFEV	223	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19982
...ERPFEV	223	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130851
...ERPFEV	223	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130852
...NEDRRS	306	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61469
...NEDRRS	306	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt92324
...EAAGAM	374	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19948
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19985
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25108
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21825
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21876
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23957
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21714
...AGAMFL	376	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC24789
...AMFLEA	378	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5345
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19986
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25109
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21826
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23658
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21877
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23958
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC21715
...LEAIPM	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC24790
...EAIPMS	382	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13911
...EAIPMS	382	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8632
...EAIPMS	382	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31935
...EAIPMS	382	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5346
...EAIPMS	382	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16119
...KPFVFL	396	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23959
...NPTQK	418	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
TRY3_HUMAN	34	AQKT.\|.TDTS	inferred from experiment	unknown	MEROPS	Sahin-Toth M	Szepessy E et al.:Human mesotrypsin exhibits rest... (S01.174)	16759229,
MMP12_HUMAN	223	PFEV.\|.VKDT	inferred from experiment	unknown	MEROPS	Shapiro SD	Gronski TJ Jr et al.:Hydrolysis of a broad spectrum ... (M10.009)	9115292,
MMP11_HUMAN	374	AGAM.\|.MFLE	inferred from experiment	unknown	MEROPS	Weiss SJ	Pei D et al.:Hydrolytic inactivation of a br... (M10.007)	7523394,
MMP12_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS		Merops MMP12_HUMAN -> A1AT_HUMAN @376
MMP9_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Travis J	Nelson D et al.:Inactivation of alpha1-proteina... (M10.001)	9657883,
MMP25_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Pei D	Nie J et al.:Rapid inactivation of alpha-1-p... (M10.024)	15149845,
MMP26_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Sang QX	Park HI et al.:Peptide substrate specificities... (M10.029)	12119297,
MMP7_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Senior RM	Sires UI et al.:Matrilysin is much more efficie... (M10.008)	7980522,
MMP1_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Travis J	Nelson D et al.:Inactivation of alpha1-proteina... (M10.001)	9657883,
MMP8_HUMAN	376	AMFL.\|.LEAI	inferred from experiment	unknown	MEROPS	Tschesche H	Knauper V et al.:Inactivation of human plasma al... (M10.002)	2159425,
CATL1_HUMAN	378	FLEA.\|.AIPM	inferred from experiment	unknown	MEROPS	Mason RW	Johnson DA et al.:Cathepsin L inactivates alpha 1... (C01.032)	3490478,
MMP12_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Senior RM	Banda MJ et al.:Alpha 1-proteinase inhibitor is... (M10.009)	3257965,
MMP9_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Travis J	Nelson D et al.:Inactivation of alpha1-proteina... (M10.001)	9657883,
MMP25_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Pei D	Nie J et al.:Rapid inactivation of alpha-1-p... (M10.024)	15149845,
MMP3_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Travis J	Nelson D et al.:Inactivation of alpha1-proteina... (M10.001)	9657883,
MMP26_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Sang QX	Park HI et al.:Peptide substrate specificities... (M10.029)	12119297,
MMP7_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS		Merops MMP7_HUMAN -> A1AT_HUMAN @381
MMP1_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Weiss SJ	Desrochers PE et al.:Interstitial collagenase (matri... (M10.001)	1645757,
MMP8_HUMAN	381	AIPM.\|.MSIP	inferred from experiment	unknown	MEROPS	Tschesche H	Knauper V et al.:Inactivation of human plasma al... (M10.002)	2159425,
GRAM_HUMAN	382	IPMS.\|.SIPP	inferred from experiment	unknown	MEROPS	Craik CS	Mahrus S et al.:Granzyme M is a regulatory prot... (S01.131)	15494398,
ELNE_HUMAN	382	IPMS.\|.SIPP	inferred from experiment	unknown	MEROPS	Travis J	Nelson D et al.:Purification and characterizati... (S01.131)	10212191,
TRY3_HUMAN	382	IPMS.\|.SIPP	inferred from experiment	unknown	MEROPS	Sahin-Toth M	Szepessy E et al.:Human mesotrypsin exhibits rest... (S01.174)	16759229,
CATL1_HUMAN	382	IPMS.\|.SIPP	inferred from experiment	unknown	MEROPS	Mason RW	Johnson DA et al.:Cathepsin L inactivates alpha 1... (C01.032)	3490478,
KLK3_HUMAN	382	IPMS.\|.SIPP	inferred from experiment	unknown	MEROPS	Stenman UH	Zhang WM et al.:Prostate-specific antigen forms... (S01.162)	9316649,
MMP7_HUMAN	396	FVFL.\|.LMIE	inferred from experiment	unknown	MEROPS	Senior RM	Sires UI et al.:Matrilysin is much more efficie... (M10.008)	7980522,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)