P06733: Alpha-enolase

General Information

Protein names	- Alpha-enolase - 4.2.1.11 {ECO:0000269\|PubMed:1369209} - 2-phospho-D-glycerate hydro-lyase - C-myc promoter-binding protein - Enolase 1 - MBP-1 - MPB-1 - Non-neural enolase - NNE - Phosphopyruvate hydratase - Plasminogen-binding protein
Gene names	ENO1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P06733

43 N-termini

21 C-termini

22 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK
        70         80         90        100        110        120
GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK
       130        140        150        160        170        180
AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE
       190        200        210        220        230        240
AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV
       250        260        270        280        290        300
VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD
       310        320        330        340        350        360
WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA
       370        380        390        400        410        420
QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK
       430
AKFAGRNFRN PLAK

Isoforms

- Isoform MBP-1 of Alpha-enolase

Sequence View

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Protein Neighborhood

Domains & Features

43 N-termini - 21 C-termini - 22 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P06733-2-unknown	MSILKI...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06733-2-Acetylation	SILKIH...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06733-2-Acetylation	SILKIH...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P06733-2-Acetylation	SILKIH...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P06733-2-Acetylation	SILKIH...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P06733-13-unknown	DSRGNP...	13		inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P06733-24-unknown	LFTSKG...	24		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11081
P06733-33-	AAVPSG...	33			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-33-	AAVPSG...	33			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06733-40-	STGIYE...	40			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-40-	STGIYE...	40			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-58-unknown	MGKGVS...	58		inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P06733-81-	KLNVTE...	81			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P06733-81-	KLNVTE...	81			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P06733-81-	KLNVTE...	81			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P06733-81-unknown	KLNVTE...	81			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P06733-94-	MIEMDG...	94			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P06733-94-unknown	MIEMDG...	94		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt73940
P06733-137-unknown	LAGNSE...	137		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3497
P06733-137-unknown	LAGNSE...	137		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138671
P06733-140-unknown	NSEVIL...	140		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3499
P06733-140-unknown	NSEVIL...	140		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138912
P06733-141-unknown	SEVILP...	141		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18523
P06733-141-unknown	SEVILP...	141		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138992
P06733-142-unknown	EVILPV...	142		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18525
P06733-142-unknown	EVILPV...	142		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt139081
P06733-155-	GGSHAG...	155			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-155-	GGSHAG...	155			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-155-	GGSHAG...	155			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P06733-155-	GGSHAG...	155			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P06733-155-	GGSHAG...	155			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06733-155-unknown	GGSHAG...	155		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt166509
P06733-157-unknown	SHAGNK...	157		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt166512
P06733-157-	SHAGNK...	157			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-157-	SHAGNK...	157			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-167-unknown	EFMILP...	167		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18527
P06733-167-unknown	EFMILP...	167		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140884
P06733-170-unknown	ILPVGA...	170		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3501
P06733-170-unknown	ILPVGA...	170		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt141133
P06733-204-	ATNVGD...	204			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-204-	ATNVGD...	204			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-204-	ATNVGD...	204			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06733-204-unknown	ATNVGD...	204		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3503
P06733-204-unknown	ATNVGD...	204		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143017
P06733-209-unknown	DEGGFA...	209		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17152
P06733-209-unknown	DEGGFA...	209		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7207
P06733-209-unknown	DEGGFA...	209		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18529
P06733-209-unknown	DEGGFA...	209		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143258
P06733-212-unknown	GFAPNI...	212		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176499
P06733-212-	GFAPNI...	212			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P06733-214-unknown	APNILE...	214		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17154
P06733-214-unknown	APNILE...	214		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143496
P06733-229-unknown	TAIGKA...	229		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5413
P06733-229-unknown	TAIGKA...	229		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144105
P06733-229-unknown	TAIGKA...	229		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06733-229-unknown	TAIGKA...	229		inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P06733-244-unknown	MDVAAS...	244		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5415
P06733-244-unknown	MDVAAS...	244		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144773
P06733-246-unknown	VAASEF...	246		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7209
P06733-246-unknown	VAASEF...	246		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144844
P06733-282-unknown	SFIKDY...	282		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt177739
P06733-282-	SFIKDY...	282			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06733-310-	SAGIQV...	310			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-310-	SAGIQV...	310			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-310-	SAGIQV...	310			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06733-310-unknown	SAGIQV...	310		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3505
P06733-310-unknown	SAGIQV...	310		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147246
P06733-312-unknown	GIQVVG...	312		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3507
P06733-312-unknown	GIQVVG...	312		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147326
P06733-312-	GIQVVG...	312			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06733-312-	GIQVVG...	312			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P06733-312-	GIQVVG...	312			Subtiligase Based Positive Selection		Wells	THP_untreated	23264352
P06733-315-unknown	VVGDDL...	315		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3509
P06733-315-unknown	VVGDDL...	315		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147448
P06733-347-unknown	IGSVTE...	347		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17156
P06733-347-unknown	IGSVTE...	347		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148571
P06733-349-unknown	SVTESL...	349		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17158
P06733-349-unknown	SVTESL...	349		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148667
P06733-364-	GWGVMV...	364			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06733-364-unknown	GWGVMV...	364		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt166514
P06733-413-	IEEELG...	413			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P06733-413-unknown	IEEELG...	413			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P06733-413-unknown	IEEELG...	413		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt161506

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...TVEVDL	23	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11081
...RHIADL	136	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3497
...RHIADL	136	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121725
...ADLAGN	139	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3499
...ADLAGN	139	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121977
...DLAGNS	140	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18523
...DLAGNS	140	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt122061
...LAGNSE	141	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18525
...LAGNSE	141	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt122152
...KLAMQE	166	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18527
...KLAMQE	166	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt124025
...MQEFMI	169	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3501
...MQEFMI	169	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt124289
...KYGKDA	203	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3503
...KYGKDA	203	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126227
...ATNVGD	208	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17152
...ATNVGD	208	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7207
...ATNVGD	208	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18529
...ATNVGD	208	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126488
...DEGGFA	213	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17154
...DEGGFA	213	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126730
...LELLKT	228	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5413
...LELLKT	228	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127356
...KVVIGM	243	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5415
...KVVIGM	243	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128048
...VIGMDV	245	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7209
...VIGMDV	245	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128120
...QKFTAS	309	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3505
...QKFTAS	309	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130589
...FTASAG	311	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3507
...FTASAG	311	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130669
...SAGIQV	314	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3509
...SAGIQV	314	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130798
...LKVNQI	346	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17156
...LKVNQI	346	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt131945
...VNQIGS	348	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17158
...VNQIGS	348	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132042
...FRNPLA	432	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8173
...FRNPLA	432	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt134146
...RNPLAK	433	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8175
...RNPLAK	433	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt134170
...NPLAK	434	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...NPLAK	434	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt69558

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	23	EVDL.\|.LFTS	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CATB_HUMAN	136	IADL.\|.LAGN	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	139	LAGN.\|.NSEV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	140	AGNS.\|.SEVI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	141	GNSE.\|.EVIL	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	166	AMQE.\|.EFMI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATB_HUMAN	169	EFMI.\|.ILPV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	203	GKDA.\|.ATNV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	208	NVGD.\|.DEGG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	208	NVGD.\|.DEGG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	208	NVGD.\|.DEGG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	213	GGFA.\|.APNI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	228	LLKT.\|.TAIG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	243	VIGM.\|.MDVA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	245	GMDV.\|.VAAS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	309	FTAS.\|.SAGI	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	311	ASAG.\|.GIQV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	314	GIQV.\|.VVGD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	346	VNQI.\|.IGSV	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	348	QIGS.\|.SVTE	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATZ_HUMAN	432	NPLA.\|.AK	inferred from experiment	unknown	MEROPS	Kos J	Obermajer N et al.:Cathepsin X cleaves the C-termi... (C01.013)	19433310,
CATZ_HUMAN	433	PLAK.\|.K	inferred from experiment	unknown	MEROPS	Kos J	Obermajer N et al.:Cathepsin X cleaves the C-termi... (C01.013)	19433310,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)