P00749: Urokinase-type plasminogen activator

General Information

Protein names	- Urokinase-type plasminogen activator - U-plasminogen activator - uPA - 3.4.21.73 - Urokinase-type plasminogen activator long chain A - Urokinase-type plasminogen activator short chain A - Urokinase-type plasminogen activator chain B
Gene names	PLAU
Organism	Homo sapiens
Protease Family	S01.231
Protease ID	S01.231
Chromosome location
UniProt ID	P00749

16 N-termini

18 C-termini

27 Cleavages

25 Substrates

Sequence

        10         20         30         40         50         60
MRALLARLLL CVLVVSDSKG SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ
        70         80         90        100        110        120
HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN
       130        140        150        160        170        180
YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII
       190        200        210        220        230        240
GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG
       250        260        270        280        290        300
RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL
       310        320        330        340        350        360
PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML
       370        380        390        400        410        420
CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR
       430
SHTKEENGLA L

Isoforms

- Isoform 2 of Urokinase-type plasminogen activator

Sequence View

Name	Sequence	Position	Evidence type	Method	Source (database)	Evidence name
P00749-21-unknown	SNELHQ...	21	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
P00749-26-unknown	QVPSNC...	26	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5324
P00749-44-unknown	YFSNIH...	44	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC32073
P00749-44-unknown	YFSNIH...	44	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt137334
P00749-59-unknown	GQHCEI...	59	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5325
P00749-59-unknown	GQHCEI...	59	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt138532
P00749-64-unknown	IDKSKT...	64	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5327
P00749-64-unknown	IDKSKT...	64	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt138929
P00749-80-unknown	GKASTD...	80	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5329
P00749-80-unknown	GKASTD...	80	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt140200
P00749-156-unknown	KPSSPP...	156	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
P00749-156-unknown	KPSSPP...	156	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27443
P00749-156-unknown	KPSSPP...	156	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt115970
P00749-164-unknown	LKFQCG...	164	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23655
P00749-164-unknown	LKFQCG...	164	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23955
P00749-164-unknown	LKFQCG...	164	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt144598
P00749-177-unknown	FKIIGG...	177	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31734
P00749-177-unknown	FKIIGG...	177	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt145167
P00749-179-unknown	IIGGEF...	179	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16404
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16686
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27445
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15203
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31607
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4743
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16030
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC701
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5331
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31965
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16117
P00749-179-unknown	IIGGEF...	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3324
P00749-179-unknown	IIGGEF...	179	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt115989
P00749-180-unknown	IGGEFT...	180	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8629
P00749-180-unknown	IGGEFT...	180	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt145282
P00749-182-unknown	GEFTTI...	182	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5333
P00749-182-unknown	GEFTTI...	182	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt145352
P00749-186-unknown	TIENQP...	186	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5335
P00749-186-unknown	TIENQP...	186	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt145511
P00749-244-unknown	LNSNTQ...	244	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15859
P00749-244-unknown	LNSNTQ...	244	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt147645
P00749-411-unknown	RVSHFL...	411	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5337
P00749-411-unknown	RVSHFL...	411	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt151909
P00749-414-unknown	HFLPWI...	414	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5339
P00749-414-unknown	HFLPWI...	414	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt151959

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...SNELHQ	25	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5324
...CVSNKY	43	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC32073
...PKKFGG	58	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5325
...PKKFGG	58	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121580
...GQHCEI	63	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5327
...GQHCEI	63	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121996
...GHFYRG	79	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5329
...GHFYRG	79	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt123314
...QTYHAH	106	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000474318
...QTYHAH	106	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt110447
...CADGKK	155	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27443
...CADGKK	155	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127499
...SPPEEL	163	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23655
...SPPEEL	163	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC23955
...SPPEEL	163	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127865
...TLRPRF	176	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31734
...TLRPRF	176	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128455
...LRPRFK	177	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...LRPRFK	177	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt91822
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16404
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16686
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27445
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15203
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31607
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4743
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16030
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC701
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5331
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC31965
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16117
...RPRFKI	178	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3324
...RPRFKI	178	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128490
...PRFKII	179	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8629
...PRFKII	179	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128573
...FKIIGG	181	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5333
...FKIIGG	181	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128644
...GGEFTT	185	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5335
...GGEFTT	185	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt128808
...LGRSRL	243	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15859
...LGRSRL	243	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130999
...PGVYTR	410	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5337
...PGVYTR	410	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135317
...YTRVSH	413	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5339
...YTRVSH	413	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135367
...NGLAL	431	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...NGLAL	431	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt89579

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL1_HUMAN	25	ELHQ.\|.QVPS	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
UROK_HUMAN	43	SNKY.\|.YFSN	inferred from experiment	unknown	MEROPS		Merops UROK_HUMAN -> UROK_HUMAN @43
CATL1_HUMAN	58	KFGG.\|.GQHC	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
CATL1_HUMAN	63	HCEI.\|.IDKS	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
CATL1_HUMAN	79	FYRG.\|.GKAS	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
PLMN_HUMAN	155	DGKK.\|.KPSS	inferred from experiment	unknown	MEROPS		Merops PLMN_HUMAN -> UROK_HUMAN @155
MMP3_HUMAN	163	PEEL.\|.LKFQ	inferred from experiment	unknown	MEROPS		Merops MMP3_HUMAN -> UROK_HUMAN @163
MMP7_HUMAN	163	PEEL.\|.LKFQ	inferred from experiment	unknown	MEROPS	Ryan JM	Marcotte PA et al.:The matrix metalloproteinase pu... (M10.008)	1629180,
THRB_HUMAN	176	RPRF.\|.FKII	inferred from experiment	unknown	MEROPS		Merops THRB_HUMAN -> UROK_HUMAN @176
KLK5_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Goettig P	Debela M et al.:Structures and specificity of t... (S01.251)	18627343,
KLKB1_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops KLKB1_HUMAN -> UROK_HUMAN @178
PLMN_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops PLMN_HUMAN -> UROK_HUMAN @178
HEPS_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Kirchhofer D	Moran P et al.:Pro-urokinase-type plasminogen ... (S01.224)	16908524,
ST14_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Uhland K	Uhland K et al.:Matriptase and its putative rol... (S01.302)	17131055,
CATG_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops CATG_HUMAN -> UROK_HUMAN @178
KLK2_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops KLK2_HUMAN -> UROK_HUMAN @178
BSSP4_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Stevens RL	Yasuda S et al.:Urokinase-type plasminogen acti... (S01.252)	15701722,
CATL1_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
TRYB1_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops TRYB1_HUMAN -> UROK_HUMAN @178
KLK3_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS		Merops KLK3_HUMAN -> UROK_HUMAN @178
CATB_HUMAN	178	RFKI.\|.IIGG	inferred from experiment	unknown	MEROPS	Graeff H	Kobayashi H et al.:Cathepsin B efficiently activat... (C01.060)	1900515,
ELNE_HUMAN	179	FKII.\|.IGGE	inferred from experiment	unknown	MEROPS		Merops ELNE_HUMAN -> UROK_HUMAN @179
CATL1_HUMAN	181	IIGG.\|.GEFT	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
CATL1_HUMAN	185	EFTT.\|.TIEN	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
KLK13_HUMAN	243	RSRL.\|.LNSN	inferred from experiment	unknown	MEROPS	Diamandis EP	Borgono CA et al.:Defining the extended substrate... (S01.306)	17976015,
CATL1_HUMAN	410	VYTR.\|.RVSH	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,
CATL1_HUMAN	413	RVSH.\|.HFLP	inferred from experiment	unknown	MEROPS	Graeff H	Goretzki L et al.:Effective activation of the pro... (C01.032)	1551416,

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
UROK-Isoform2_HUMAN	26	SNKY.\|.YFSN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32073
CCL14_HUMAN	27	SSRG.\|.GPYH	inferred from experiment	unknown	MEROPS	Parmentier M	Vakili J et al.:Urokinase plasminogen activator... (S01.233)	11544332,
UROK_HUMAN	43	SNKY.\|.YFSN	inferred from experiment	unknown	MEROPS		Merops UROK_HUMAN -> UROK_HUMAN @43
UPAR_HUMAN	105	SGRA.\|.AVTY	inferred from experiment	unknown	MEROPS	Sidenius N	Andolfo A et al.:Metalloproteases cleave the uro... (S01.131)	12195704,
UPAR-Isoform3_HUMAN	105	SGRA.\|.AVTY	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32088
UPAR-Isoform2_HUMAN	105	SGRA.\|.AVTY	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32088
UPAR_HUMAN	111	YSRS.\|.SRYL	inferred from experiment	unknown	MEROPS	Sidenius N	Andolfo A et al.:Metalloproteases cleave the uro... (S01.131)	12195704,
UPAR-Isoform3_HUMAN	111	YSRS.\|.SRYL	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32091
UPAR-Isoform2_HUMAN	111	YSRS.\|.SRYL	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32091
HABP2-Isoform2_HUMAN	287	IKRI.\|.IYGG	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32094
HABP2_HUMAN	313	IKRI.\|.IYGG	inferred from experiment	unknown	MEROPS	Romisch J	Kannemeier C et al.:Factor VII and single-chain pla... (S01.231)	11432747,
PGCA_HUMAN	397	GSVI.\|.ILTV	inferred from experiment	unknown	MEROPS	Okada Y	Fosang AJ et al.:Membrane-type 1 MMP (MMP-14) cl... (M10.013)	9688535,
PGCA-Isoform3_HUMAN	397	GSVI.\|.ILTV	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32077
PGCA-Isoform2_HUMAN	397	GSVI.\|.ILTV	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32077
ITA6-Isoform7_HUMAN	475	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
HGF-Isoform3_HUMAN	489	QLRV.\|.VVNG	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32075
HGF_HUMAN	494	QLRV.\|.VVNG	inferred from experiment	unknown	MEROPS	Michalopoulos GK	Mars WM et al.:Activation of hepatocyte growth... (S01.231)	8362987,
PLMN_HUMAN	580	PGRV.\|.VVGG	inferred from experiment	unknown	MEROPS	Shah RJ	Robbins KC et al.:The peptide chains of human pla... (S01.231)	4226004,
ITA6-Isoform5_HUMAN	589	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
ITA6-Isoform4_HUMAN	589	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
ITA6-Isoform3_HUMAN	594	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
ITA6-Isoform2_HUMAN	594	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
ITA6_HUMAN	633	SSRR.\|.RRVN	inferred from experiment	unknown	MEROPS	Cress AE	Pawar SC et al.:Integrin alpha6 cleavage: a nov... (S01.231)	17303120,
ITA6-Isoform9_HUMAN	633	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080
ITA6-Isoform6_HUMAN	633	SSRR.\|.RRVN	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC32080

TopFIND

P00749: Urokinase-type plasminogen activator

General Information

16

N-termini

18

C-termini

27

Cleavages

25

Substrates

Sequence

Isoforms

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Protein Neighborhood

Domains & Features

16 N-termini - 18 C-termini - 27 Cleavages - 25 Substrates

N-termini

C-termini

Cleavages

Substrates